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hbegf  (Bioss)
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Hbegf, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Schematic of the protocol used to transfect HBV-infected HepG2-hNTCP cells with ABE-encoding mRNA and HBs/POL ORF targeting gRNA (gS1, gS2, or gS3). (B) The levels of extracellular HBsAg were determined by ELISA. (C) intracellular total HBV DNA and (D) cccDNA levels were quantified by qPCR. (E) Percentage of A-to-G editing of HBs ORF by gS1-gS3 and control gRNA was detected on exonucleases I/III-treated samples and total extracted DNA samples, respectively. control: non-HBV targeting gRNA. Data are represented as mean±SEM. (F) The levels of intracellular HBsAg were determined by Western blot <t>using</t> <t>anti-HBs</t> monoclonal (H166, Abbot) or <t>polyclonal</t> antibody <t>(NB100-62652,</t> NovusBio). Ponceau staining of total protein was used to control protein loading.
Polyclonal Anti Hbs, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Schematic of the protocol used to transfect HBV-infected HepG2-hNTCP cells with ABE-encoding mRNA and HBs/POL ORF targeting gRNA (gS1, gS2, or gS3). (B) The levels of extracellular HBsAg were determined by ELISA. (C) intracellular total HBV DNA and (D) cccDNA levels were quantified by qPCR. (E) Percentage of A-to-G editing of HBs ORF by gS1-gS3 and control gRNA was detected on exonucleases I/III-treated samples and total extracted DNA samples, respectively. control: non-HBV targeting gRNA. Data are represented as mean±SEM. (F) The levels of intracellular HBsAg were determined by Western blot <t>using</t> <t>anti-HBs</t> monoclonal (H166, Abbot) or <t>polyclonal</t> antibody <t>(NB100-62652,</t> NovusBio). Ponceau staining of total protein was used to control protein loading.
Anti Hbs Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti hbs antibody/product/Bioss
Average 94 stars, based on 1 article reviews
anti hbs antibody - by Bioz Stars, 2026-05
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(A) Schematic of the protocol used to transfect HBV-infected HepG2-hNTCP cells with ABE-encoding mRNA and HBs/POL ORF targeting gRNA (gS1, gS2, or gS3). (B) The levels of extracellular HBsAg were determined by ELISA. (C) intracellular total HBV DNA and (D) cccDNA levels were quantified by qPCR. (E) Percentage of A-to-G editing of HBs ORF by gS1-gS3 and control gRNA was detected on exonucleases I/III-treated samples and total extracted DNA samples, respectively. control: non-HBV targeting gRNA. Data are represented as mean±SEM. (F) The levels of intracellular HBsAg were determined by Western blot <t>using</t> <t>anti-HBs</t> monoclonal (H166, Abbot) or <t>polyclonal</t> antibody <t>(NB100-62652,</t> NovusBio). Ponceau staining of total protein was used to control protein loading.
Goat Polyclonal Anti Hbs Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat polyclonal anti hbs antibody/product/Bioss
Average 94 stars, based on 1 article reviews
goat polyclonal anti hbs antibody - by Bioz Stars, 2026-05
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Bioss polyclonal goat anti hbs antibody
(A) Schematic of the protocol used to transfect HBV-infected HepG2-hNTCP cells with ABE-encoding mRNA and HBs/POL ORF targeting gRNA (gS1, gS2, or gS3). (B) The levels of extracellular HBsAg were determined by ELISA. (C) intracellular total HBV DNA and (D) cccDNA levels were quantified by qPCR. (E) Percentage of A-to-G editing of HBs ORF by gS1-gS3 and control gRNA was detected on exonucleases I/III-treated samples and total extracted DNA samples, respectively. control: non-HBV targeting gRNA. Data are represented as mean±SEM. (F) The levels of intracellular HBsAg were determined by Western blot <t>using</t> <t>anti-HBs</t> monoclonal (H166, Abbot) or <t>polyclonal</t> antibody <t>(NB100-62652,</t> NovusBio). Ponceau staining of total protein was used to control protein loading.
Polyclonal Goat Anti Hbs Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal goat anti hbs antibody/product/Bioss
Average 94 stars, based on 1 article reviews
polyclonal goat anti hbs antibody - by Bioz Stars, 2026-05
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(A) Schematic of the protocol used to transfect HBV-infected HepG2-hNTCP cells with ABE-encoding mRNA and HBs/POL ORF targeting gRNA (gS1, gS2, or gS3). (B) The levels of extracellular HBsAg were determined by ELISA. (C) intracellular total HBV DNA and (D) cccDNA levels were quantified by qPCR. (E) Percentage of A-to-G editing of HBs ORF by gS1-gS3 and control gRNA was detected on exonucleases I/III-treated samples and total extracted DNA samples, respectively. control: non-HBV targeting gRNA. Data are represented as mean±SEM. (F) The levels of intracellular HBsAg were determined by Western blot <t>using</t> <t>anti-HBs</t> monoclonal (H166, Abbot) or <t>polyclonal</t> antibody <t>(NB100-62652,</t> NovusBio). Ponceau staining of total protein was used to control protein loading.
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Bioss rrid ab 628073 rabbit anti hb egf bioss bs 3576r
(A) Schematic of the protocol used to transfect HBV-infected HepG2-hNTCP cells with ABE-encoding mRNA and HBs/POL ORF targeting gRNA (gS1, gS2, or gS3). (B) The levels of extracellular HBsAg were determined by ELISA. (C) intracellular total HBV DNA and (D) cccDNA levels were quantified by qPCR. (E) Percentage of A-to-G editing of HBs ORF by gS1-gS3 and control gRNA was detected on exonucleases I/III-treated samples and total extracted DNA samples, respectively. control: non-HBV targeting gRNA. Data are represented as mean±SEM. (F) The levels of intracellular HBsAg were determined by Western blot <t>using</t> <t>anti-HBs</t> monoclonal (H166, Abbot) or <t>polyclonal</t> antibody <t>(NB100-62652,</t> NovusBio). Ponceau staining of total protein was used to control protein loading.
Rrid Ab 628073 Rabbit Anti Hb Egf Bioss Bs 3576r, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Schematic of the protocol used to transfect HBV-infected HepG2-hNTCP cells with ABE-encoding mRNA and HBs/POL ORF targeting gRNA (gS1, gS2, or gS3). (B) The levels of extracellular HBsAg were determined by ELISA. (C) intracellular total HBV DNA and (D) cccDNA levels were quantified by qPCR. (E) Percentage of A-to-G editing of HBs ORF by gS1-gS3 and control gRNA was detected on exonucleases I/III-treated samples and total extracted DNA samples, respectively. control: non-HBV targeting gRNA. Data are represented as mean±SEM. (F) The levels of intracellular HBsAg were determined by Western blot <t>using</t> <t>anti-HBs</t> monoclonal (H166, Abbot) or <t>polyclonal</t> antibody <t>(NB100-62652,</t> NovusBio). Ponceau staining of total protein was used to control protein loading.
Hb Egf, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Schematic of the protocol used to transfect HBV-infected HepG2-hNTCP cells with ABE-encoding mRNA and HBs/POL ORF targeting gRNA (gS1, gS2, or gS3). (B) The levels of extracellular HBsAg were determined by ELISA. (C) intracellular total HBV DNA and (D) cccDNA levels were quantified by qPCR. (E) Percentage of A-to-G editing of HBs ORF by gS1-gS3 and control gRNA was detected on exonucleases I/III-treated samples and total extracted DNA samples, respectively. control: non-HBV targeting gRNA. Data are represented as mean±SEM. (F) The levels of intracellular HBsAg were determined by Western blot using anti-HBs monoclonal (H166, Abbot) or polyclonal antibody (NB100-62652, NovusBio). Ponceau staining of total protein was used to control protein loading.

Journal: bioRxiv

Article Title: Adenine Base Editing Potently Suppresses Hepatitis B Surface Antigen Expression and Inhibits Hepatitis D Virus Release

doi: 10.64898/2026.02.06.704371

Figure Lengend Snippet: (A) Schematic of the protocol used to transfect HBV-infected HepG2-hNTCP cells with ABE-encoding mRNA and HBs/POL ORF targeting gRNA (gS1, gS2, or gS3). (B) The levels of extracellular HBsAg were determined by ELISA. (C) intracellular total HBV DNA and (D) cccDNA levels were quantified by qPCR. (E) Percentage of A-to-G editing of HBs ORF by gS1-gS3 and control gRNA was detected on exonucleases I/III-treated samples and total extracted DNA samples, respectively. control: non-HBV targeting gRNA. Data are represented as mean±SEM. (F) The levels of intracellular HBsAg were determined by Western blot using anti-HBs monoclonal (H166, Abbot) or polyclonal antibody (NB100-62652, NovusBio). Ponceau staining of total protein was used to control protein loading.

Article Snippet: Immunodetection was performed using primary antibodies including monoclonal anti-HBs (Abbott H166 mouse), polyclonal anti-HBs (Novus biologicals NB100-62652 rabbit), anti-Ku80 (ab119935, mouse, Abcam), anti-HDV (homemade, mouse), followed by incubation with horseradish peroxidase (HRP) or fluorophore-conjugated secondary antibodies.

Techniques: Infection, Enzyme-linked Immunosorbent Assay, Control, Western Blot, Staining